Anti-Hexokinase 1 antibody (ab150013)
Key features and details
- Rabbit polyclonal to Hexokinase 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hexokinase 1 antibody
See all Hexokinase 1 primary antibodies -
Description
Rabbit polyclonal to Hexokinase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Hexokinase 1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab134654) -
Positive control
- This antibody gave a positive signal in Hexokinase 1 Recombinant Protein and Human Testis tissue lysate as well as the following whole cell lysates: HeLa; MCF7; K562.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatRabbitHorseCowDogChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).Target
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Tissue specificity
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific. -
Pathway
Carbohydrate metabolism; hexose metabolism. -
Involvement in disease
Hexokinase deficiency
Neuropathy, hereditary motor and sensory, Russe type -
Sequence similarities
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
Domain
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
Cellular localization
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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Database links
- Entrez Gene: 3098 Human
- Entrez Gene: 15275 Mouse
- Entrez Gene: 25058 Rat
- Omim: 142600 Human
- SwissProt: P19367 Human
- SwissProt: P17710 Mouse
- SwissProt: P05708 Rat
- Unigene: 370365 Human
see all -
Alternative names
- BB404130 antibody
- Brain form hexokinase antibody
- dea antibody
see all
Images
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Western blot - Anti-Hexokinase 1 antibody (ab150013)All lanes : Anti-Hexokinase 1 antibody (ab150013) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Additional bands at: 46 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Western blot - Anti-Hexokinase 1 antibody (ab150013)Anti-Hexokinase 1 antibody (ab150013) at 1/1 dilution +Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 1 minute
Datasheets and documents
References (0)
ab150013 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Hexokinase 1 antibody (ab150013)All lanes : Anti-Hexokinase 1 antibody (ab150013) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Additional bands at: 46 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
-
Western blot - Anti-Hexokinase 1 antibody (ab150013)Anti-Hexokinase 1 antibody (ab150013) at 1/1 dilution +
Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 1 minute
