Anti-CYP1A1 antibody (ab235185)
Key features and details
- Rabbit polyclonal to CYP1A1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP1A1 antibody
See all CYP1A1 primary antibodies -
Description
Rabbit polyclonal to CYP1A1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- ICC/IF: HepG2 cells. IHC-P: Human kidney tissue. WB: CYP1A1 overexpressing lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CYP1A1 antibody (ab235185) at 1/1000 dilution
Lane 1 : CYP1A1 overexpressing lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) lysate
Secondary
All lanes : Rabbit IgG (H&L) secondary antibody peroxidase conjugated Pre-adsorbed at 1/70000 dilution
Predicted band size: 58 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: Proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an anti-microbial agent.
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Formalin/PFA-fixed paraffin-embedded human kidney tissue stained for CYP1A1 with ab235185 at a 1/100 dilution in immunohistochemical analysis. Secondary antibody is an anti-Rabbit poly-HRP-IgG.
Incubated with primary antibody for 30 minutes at room temperature, followed by DAB staining.
Counterstained with hematoxylin. HIER using citrate buffer for 20 minutes.
A. Human kidney tissue counterstained with Hematoxilyn-Eosin.
B. Human kidney tissue stained for Cytochrome P450 1A1 (20X).
C. Human kidney tissue stained for Cytochrome P450 1A1 (40X).
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP1A1 using ab235185 at 15 µg/ml in ICC/IF.
Cells were fixed in 4% PFA and permeabilized using 0.3% Triton X-100, then incubated with the primary antibody overnight at 4°C. Secondary antibody is a Donkey anti-Rabbit IgG DyLight™ 488 (Preadsorbed), at 5 µg/ml for 1 hour at room temperature.
Panel A: DAPI (blue)
Panel B: ab235185 + secondary antibody (green)
Panel C: Actin-Phalloidin 555 (red)
Panel D: Secondary antibody only
Panel E: Merge A+B
Panel F: Merge A+B+C