Anti-CYP1A1 antibody (ab124295)
Key features and details
- Rabbit polyclonal to CYP1A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP1A1 antibody
See all CYP1A1 primary antibodies -
Description
Rabbit polyclonal to CYP1A1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human CYP1A1 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab155750) -
Positive control
- This antibody gave a positive signal in both Human Lung and Placenta tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications DogPigChimpanzeeMacaque monkeyChinese hamsterOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 58 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 58 kDa).Target
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Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. -
Tissue specificity
Lung, lymphocytes and placenta. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1543 Human
- Entrez Gene: 403103 Pig
- Omim: 108330 Human
- SwissProt: P04798 Human
- Unigene: 72912 Human
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Alternative names
- AHH antibody
- AHRR antibody
- Aryl hydrocarbon hydroxylase antibody
see all
Images
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Western blot - Anti-CYP1A1 antibody (ab124295)All lanes : Anti-CYP1A1 antibody (ab124295) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124295 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab124295 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-CYP1A1 antibody (ab124295)All lanes : Anti-CYP1A1 antibody (ab124295) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124295 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
