Human Legumain ELISA Kit (ab267603)
Key features and details
- Sensitivity: 28.7 pg/ml
- Range: 28.67 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Legumain ELISA Kit
See all Legumain kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
28.7 pg/ml -
Range
28.67 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103.4 69% - 129% Plasma 109.1 82% - 144% Cell culture media 124.4 118% - 133% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Legumain ELISA Kit is designed for the quantitative determination of Legumain in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Legumain coated on a 96-well plate. Standards and samples are pipetted into the wells and Legumain present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Legumain antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Legumain bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human 2B4 (CD244), ADAM-9, Angiopoietin-2, APRIL, BMP-2, BMP-9, C5a, Cathepsin L, CD200, CD97, Chemerin, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha / betaR2, IGF-2, IGF-2 R, IL-1 R6 (IL-1 Rrp2), IL-24, IL-33 (IL-1 F11), Kallikrein 14.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human Legumain coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Legumain Detection Antibody 2 vials Human Legumain Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. -
Tissue specificity
Ubiquitous. Particularly abundant in kidney, heart and placenta. -
Sequence similarities
Belongs to the peptidase C13 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- AEP
- Asparaginyl endopeptidase
- cysteine 1
see all -
Database links
- Entrez Gene: 5641 Human
- Omim: 602620 Human
- SwissProt: Q99538 Human
- Unigene: 726036 Human
Images
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Human Legumain ELISA kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.