Anti-FEN1 antibody [EPR4459(2)] (ab133311)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4459(2)] to FEN1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-FEN1 antibody [EPR4459(2)]
See all FEN1 primary antibodies -
Description
Rabbit monoclonal [EPR4459(2)] to FEN1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human FEN1 aa 100-200. The exact sequence is proprietary.
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Positive control
- Jurkat and HeLa cell lysates; Human breast and endometrial adenocarcinoma tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4459(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FEN1 antibody [EPR4459(2)] (ab133311) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
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Immunohistochemical analysis of paraffin-embedded Human breast tissue labelled with ab133311 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelled with ab133311 at 1/500 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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