Anti-FEN1 antibody (ab153825)
Key features and details
- Rabbit polyclonal to FEN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FEN1 antibody
See all FEN1 primary antibodies -
Description
Rabbit polyclonal to FEN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 169 and 380 of Human FEN1 (UniProt: P39748).
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Positive control
- Recombinant Human FEN1 protein (ab95382) can be used as a positive control in WB. HepG2, NIH 3T3, JC, BCL1, Raw264.7 and PC12 whole cell lysates; A549 cells; A549 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-FEN1 antibody (ab153825) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
All lanes : Anti-FEN1 antibody (ab153825) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lane 4 : Raw264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
10% SDS-PAGE -
Anti-FEN1 antibody (ab153825) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS-PAGE -
Immunofluorescence analysis of paraformaldehyde fixed A549 cells labeling FEN1 with ab153825 at 1/200. The lower panel is merged with DNA probe.
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Immunohistochemical analysis of paraffin embedded A549 xenograft tissue labeling FEN1 with ab153825 at 1/100.