Anti-FEN1 antibody (ab17994)
Key features and details
- Rabbit polyclonal to FEN1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FEN1 antibody
See all FEN1 primary antibodies -
Description
Rabbit polyclonal to FEN1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide representing human Flap Structure-specific Endonuclease I encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of FEN1 that begins a few residues downstream from the I-domain as defined in Swiss-Prot entry P39748.
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Positive control
- IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-FEN1 antibody (ab17994)Detection of FEN1 by immunoprecipitation of whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells using NETN lysis buffer. Lane 1: Anti-FEN1 antibody (ab17994) at 6 µg/mg lysate, Lane 2: Anti-FEN1 antibody (ab17994) at 6 µg/mg lysate, Lane 3: Control IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western blot - Anti-FEN1 antibody (ab17994)ab17994 at the indicated concentrations, staining human FEN1 in (20
ab17994 at the indicated concentrations, staining human FEN1 in (20µg) HeLa cell lysate by Western blot. Secondary antibody coonjugated to HRP used at 1/40000 (for 0.015 µg/ml anti-Fen1) or 1/60000 (for 1 and 0.1µg/ml anti-Fen1). Detection was by chemiluminescence with the indicated exposure.µ g) HeLa cell lysate by Western blot. Secondary antibody coonjugated to HRP used at 1/40000 (for 0.015µ g/ml anti-Fen1) or 1/60000 (for 1 and 0.1µ g/ml anti-Fen1). Detection was by chemiluminescence with the indicated exposure. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (ab17994)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FEN1 with ab17994 at 1/500 (2µg/ml). Detection: DAB.
