Anti-V2R antibody (ab109326)
Key features and details
- Rabbit polyclonal to V2R
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-V2R antibody
See all V2R primary antibodies -
Description
Rabbit polyclonal to V2R -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive signal in HEK293 whole cell lysate as well as the following tissue lysates: Human Fetal Kidney and Mouse Kidney.
-
General notes
This product was previously labelled as AVPR V2
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications RatChimpanzeeGorillaChinese hamsterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40,43 kDa (predicted molecular weight: 40 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40,43 kDa (predicted molecular weight: 40 kDa).Target
-
Function
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. -
Tissue specificity
Kidney. -
Involvement in disease
Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD) [MIM:300539]. This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis.
Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI) [MIM:304800]; also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 554 Human
- Entrez Gene: 12000 Mouse
- Entrez Gene: 25108 Rat
- Omim: 300538 Human
- SwissProt: P30518 Human
- SwissProt: O88721 Mouse
- SwissProt: Q00788 Rat
- Unigene: 567240 Human
see all -
Alternative names
- ADHR antibody
- Antidiuretic hormone receptor antibody
- Arginine vasopressin receptor 2 antibody
see all
Images
-
Western blot - Anti-V2R antibody (ab109326)All lanes : Anti-V2R antibody (ab109326) at 1 µg/ml (Milk blocking - 5%)
Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa, 72 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Western blot - Anti-V2R antibody (ab109326)Anti-V2R antibody (ab109326) at 1 µg/ml (Milk blocking - 3%) + Kidney (Mouse) Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 12 minutesV2R contains a potential glycosylation site (SwissProt) which may explain the banding pattern observed.
Datasheets and documents
References (5)
ab109326 has been referenced in 5 publications.
- Wang F et al. Site-1 protease-derived soluble (pro)renin receptor targets vasopressin receptor 2 to enhance urine concentrating capability. JCI Insight 4:N/A (2019). PubMed: 30944256
- Luo R et al. Hydrogen sulfide upregulates renal AQP-2 protein expression and promotes urine concentration. FASEB J 33:469-483 (2019). PubMed: 30036087
- García-Arroyo FE et al. A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose. Int J Mol Sci 20:N/A (2019). PubMed: 31744099
- Czarzasta K et al. Effect of Chronic Kidney Disease on Changes in Vasopressin System Expression in the Kidney Cortex in Rats with Nephrectomy. Biomed Res Int 2018:2607928 (2018). PubMed: 30013980
- Xu W et al. Downregulating hypoxia-inducible factor-1a expression with perfluorooctyl-bromide nanoparticles reduces early brain injury following experimental subarachnoid hemorrhage in rats. Am J Transl Res 8:2114-26 (2016). PubMed: 27347319
Images
-
Western blot - Anti-V2R antibody (ab109326)All lanes : Anti-V2R antibody (ab109326) at 1 µg/ml (Milk blocking - 5%)
Lane 1 : Kidney (Human) Tissue Lysate - fetal normal tissue (ab30204)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa, 72 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
-
Western blot - Anti-V2R antibody (ab109326)Anti-V2R antibody (ab109326) at 1 µg/ml (Milk blocking - 3%) + Kidney (Mouse) Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 12 minutesV2R contains a potential glycosylation site (SwissProt) which may explain the banding pattern observed.
