Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD208 antibody [EPR24265-8] (ab271053)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24265-8] to CD208
Suitable for: IHC-P
Reacts with: Human

Product name

Anti-CD208 antibody [EPR24265-8]
See all CD208 primary antibodies
Description

Rabbit monoclonal [EPR24265-8] to CD208
Host species

Rabbit
Tested applications

Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC,IP or WB
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human spleen, tonsil, cervical carcinoma tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24265-8
Isotype

IgG

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] (ab271053)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Dendritic cells in human spleen (PMID: 23528943).The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] (ab271053)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Dendritic cells in human tonsil (PMID: 23528943).The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] (ab271053)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cervical carcinoma.The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] (ab271053)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cerebrum.The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD208 antibody [EPR24265-8] (ab271053)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling CD208 with ab271053 at 1/500 (0.938 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cardiac muscle.The section was incubated with ab271053 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-CD208 antibody [EPR24265-8] (ab271053)

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