Anti-CD62E antibody (ab18981)
Key features and details
- Rabbit polyclonal to CD62E
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD62E antibody
See all CD62E primary antibodies -
Description
Rabbit polyclonal to CD62E -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
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Positive control
- WB: Jurkat cell lysate. Flow Cyt: Jurkat cells. IHC-P: Human placenta and mouse aorta tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.15% EDTA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD62E antibody (ab18981)Anti-CD62E antibody (ab18981) at 1/1000 dilution + Jurkat cell lysate at 80 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST.
Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)ab18981 IHC staining of human placenta with E-Selectin Polyclonal antibody: Immunohistochemistry of formalin fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 5 μg/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
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Flow Cytometry - Anti-CD62E antibody (ab18981)Flow cytometric analysis of Jurkat cells using ab18981 (red) with negative controls (black and green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)ab18981 IHC staining of mouse aorta from obese diabetic mice with E-Selectin Polyclonal antibody: Immunohistochemistry of paraffin-embedded tissue after heat-induced antigen retrieval. 10-20 μg/mL of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit HRP and visualized with DAB substrate using ABC staining system.
