Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [2H12] to Bcl-XL - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Bcl-XL antibody [2H12] - BSA and Azide free
    See all Bcl-XL primary antibodies

  • Description

    Mouse monoclonal [2H12] to Bcl-XL - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon carcinoma tissue and kidney tissue. WB: Ramos, HCT 116, C6 and NIH/3T3 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252257 is the carrier-free version of ab77571. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252257 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
    Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
    All lanes : Anti-Bcl-XL antibody [2H12] (ab77571) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77571).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Bcl-XL with ab77571 at 1/100 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human kidney. The section was incubated with ab77571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77571).

  • Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
    Western blot - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
    All lanes : Anti-Bcl-XL antibody [2H12] (ab77571) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77571).

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 37 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling Bcl-XL with ab77571 at 1/100 dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human colon carcinoma. The section was incubated with ab77571 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab77571).

  • Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)
    Anti-Bcl-XL antibody [2H12] - BSA and Azide free (ab252257)

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