Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DNA PKcs antibody [EPR392] (ab133516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR392] to DNA PKcs
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-DNA PKcs antibody [EPR392]
    See all DNA PKcs primary antibodies

  • Description

    Rabbit monoclonal [EPR392] to DNA PKcs

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DNA PKcs aa 50-150. The exact sequence is proprietary.
    Database link: P78527

  • Positive control

    • WB: K562, Molt4, MCF7, SH-SY5Y, 293T and PC3 cell lysates. ICC/IF: K-562 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-DNA PKcs antibody [EPR392] (ab133516)
    Western blot - Anti-DNA PKcs antibody [EPR392] (ab133516)

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)
    Lane 2: DNA PKcs knockout HAP1 whole cell lysate (40 µg)
    Lane 3: K562 whole cell lysate (20 µg)
    Lane 4: SHSY5Y whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133516 observed at 460 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab133516 was shown to specifically react with DNA PKcs when DNA PKcs knockout samples were used. Wild-type and DNA PKcs knockout samples were subjected to SDS-PAGE. Ab133516 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-DNA PKcs antibody [EPR392] (ab133516)
    Western blot - Anti-DNA PKcs antibody [EPR392] (ab133516)
    All lanes : Anti-DNA PKcs antibody [EPR392] (ab133516) at 1/5000 dilution (Purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 469 kDa
    Observed band size: 460 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [EPR392] (ab133516)
    Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs antibody [EPR392] (ab133516)
    Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling DNA PKcs with purified ab133516 at 1:200 dilution (9.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • OI-RD Scanning - Anti-DNA PKcs antibody [EPR392] (ab133516)
    OI-RD Scanning - Anti-DNA PKcs antibody [EPR392] (ab133516)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-DNA PKcs antibody [EPR392] (ab133516)
    Western blot - Anti-DNA PKcs antibody [EPR392] (ab133516)
    All lanes : Anti-DNA PKcs antibody [EPR392] (ab133516) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Molt4 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : SH-SY5Y cell lysate
    Lane 5 : 293T cell lysate
    Lane 6 : PC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 469 kDa
    Observed band size: 460 kDa why is the actual band size different from the predicted?

  • Anti-DNA PKcs antibody [EPR392] (ab133516)
    Anti-DNA PKcs antibody [EPR392] (ab133516)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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