HRP Anti-DNA PKcs antibody [Y393] (ab195537)
![HRP Anti-DNA PKcs antibody [Y393] (ab195537)](https://www.abcam.com/ps/products/195/ab195537/Images/ab195537-356634-rabbitmonoclonaly393todnapkcshrpwesternblotknockouthap1prkdchumanlysates.jpg "HRP Anti-DNA PKcs antibody [Y393] (ab195537)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y393] to DNA PKcs
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-DNA PKcs antibody [Y393]
See all DNA PKcs primary antibodies -
Description
HRP Rabbit monoclonal [Y393] to DNA PKcs -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y393 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-DNA PKcs antibody [Y393] (ab195537)All lanes : HRP Anti-DNA PKcs antibody [Y393] (ab195537) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PRKDC knockout HAP1 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 469 kDa
Observed band size: 460 kDa why is the actual band size different from the predicted?ab195537 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in PRKDC knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PRKDC knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab195537 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-DNA PKcs antibody [Y393] (ab195537)](https://www.abcam.com/ps/products/195/ab195537/Images/ab195537-238036-anti-dna-pkcs-antibody-y393-hrp-western-blot.jpg)
Western blot - HRP Anti-DNA PKcs antibody [Y393] (ab195537)HRP Anti-DNA PKcs antibody [Y393] (ab195537) at 1/5000 dilution + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 469 kDa
Observed band size: 460 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195537 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-DNA PKcs antibody [Y393] (ab195537)](https://www.abcam.com/ps/products/195/ab195537/Images/ab195537-3-benefits-of-recombinant-antibodies.png)
HRP Anti-DNA PKcs antibody [Y393] (ab195537)
