Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)
![Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)](https://www.abcam.com/ps/products/242/ab242151/Images/ab242151-1-anti-fancafaa-antibody-epr2366761-immunohistochemistry-mouse-testis.jpg "Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23667-61] to FANCA/FAA
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-FANCA/FAA antibody [EPR23667-61]
See all FANCA/FAA primary antibodies -
Description
Rabbit monoclonal [EPR23667-61] to FANCA/FAA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseIP MouseWB MouseRat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse thymus, testis and embryo lysates; Rat testis lysate; NIH/3T3 whole cell lysate. IHC-P: Mouse testis tissue. IP: MEF whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23667-61 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling FANCA/FAA with ab242151 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 10754110, PMID: 16397136). The section was incubated with ab242151 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)](https://www.abcam.com/ps/products/242/ab242151/Images/ab242151-390880-anti-fancafaa-antibody-epr2366761-western-blot-mouse-testis-tissue-lysate-20-ug.jpg)
Western blot - Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)All lanes : Anti-FANCA/FAA antibody [EPR23667-61] (ab242151) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse embryo tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 161 kDa
Observed band size: 161 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)](https://www.abcam.com/ps/products/242/ab242151/Images/ab242151-390877-anti-fancafaa-antibody-epr2366761-western-blot-mouse-thymus-tissue-lysate-20-ug.jpg)
Western blot - Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)All lanes : Anti-FANCA/FAA antibody [EPR23667-61] (ab242151) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 161 kDa
Observed band size: 161 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissues: mouse heart, mouse lung(PMID: 10754110).
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)](https://www.abcam.com/ps/products/242/ab242151/Images/ab242151-2-anti-fancafaa-antibody-epr2366761-immunoprecipitation-mef.jpg)
Immunoprecipitation - Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)FANCA/FAA was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with ab242151 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242151 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 10 ug
Lane 2: ab242151 IP in MEF whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242151 in MEF whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
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![Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)](https://www.abcam.com/ps/products/242/ab242151/Images/ab242151-5-benefits-of-recombinant-antibodies.png)
Anti-FANCA/FAA antibody [EPR23667-61] (ab242151)