Human RRM2B (p53R2) knockout HCT116 cell lysate (ab257216)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263500 - Human RRM2B knockout HCT116 cell lysate 1 x 100µg ab255555 - Human wild-type HCT116 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10
Images
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Western blot - Human RRM2B (p53R2) knockout HCT116 cell lysate (ab257216)
Lane 1: Wild-Type HCT116 cell lysate (20µg)
Lane 2: RRM2B knockout HCT116 cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab154194 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab154194 Anti-p53R2 antibody [EPR8816] was shown to specifically react with p53R2 in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266897 (knockout cell lysate ab257216) was used. Wild-type and p53R2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab154194 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human RRM2B knockout HCT116 cell lysate (ab257216)Allele-1: 1 bp insertion in exon1
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Sanger Sequencing - Human RRM2B knockout HCT116 cell lysate (ab257216)Allele-2: Insertion of the selection cassette in exon1