Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab121238 1-4 µg/ml. Cells treated with PFA/Triton X-100.

All lanes : Anti-POLR3K antibody (ab121238) at 1/250 dilution

Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10

Lane 2 : Negative control (vector only transfected HEK293T lysate)

Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunohistochemical staining of paraffin embedded Human testis tissue, using 1/50 ab121238, shows strong nuclear positivity in cells of seminiferus ducts.