Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)
![Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)](https://www.abcam.com/ps/products/154/ab154839/Images/ab154839-383528-anti-hexokinase-1-antibody-epr10135b-western-blot-hek293t-mcf7-hepg2.jpg "Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10135(B)] to Hexokinase 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Hexokinase 1 antibody [EPR10135(B)]
See all Hexokinase 1 primary antibodies -
Description
Rabbit monoclonal [EPR10135(B)] to Hexokinase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to residues in Human Hexokinase 1 (Uniprot: P19367)
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Positive control
- WB: HEK-293T, MCF7, U-87 MG, and HeLa cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10135(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)All lanes : Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : HK1 knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDaLanes 1-4: Merged signal (red and green). Green - ab154839 observed at 102 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab154839 Anti-Hexokinase 1 antibody [EPR10135(B)] was shown to specifically react with Hexokinase 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267279 (knockout cell lysate ab257161) was used. Wild-type and Hexokinase 1 knockout samples were subjected to SDS-PAGE. ab154839 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)](https://www.abcam.com/ps/products/154/ab154839/Images/Hexokinase-1-Primary-antibodies-ab154839-1.jpg)
Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)All lanes : Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 102 kDa
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![Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)](https://www.abcam.com/ps/products/154/ab154839/Images/ab154839-3-benefits-of-recombinant-antibodies.png)
Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)
