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Anti-Rad53 antibody [EL7.E1] (ab166859)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Rad53 antibody [EL7.E1] (ab166859)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [EL7.E1] to Rad53
  • Suitable for: WB
  • Reacts with: Saccharomyces cerevisiae
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Rad53 antibody [EL7.E1]
    See all Rad53 primary antibodies
  • Description

    Mouse monoclonal [EL7.E1] to Rad53
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
    Does not react with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Rad53. Yeast RAD53 produced as a recombinant protein in E. coli.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    EL7.E1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Chk1 / Chk2
    • Cell Biology
    • Cell Cycle
    • Cell differentiation

Images

  • Western blot - Anti-Rad53 antibody [EL7.E1] (ab166859)
    Western blot - Anti-Rad53 antibody [EL7.E1] (ab166859)
    All lanes : Anti-Rad53 antibody [EL7.E1] (ab166859) at 1 µg/ml

    Lane 1 : TCA preps from S. cerevisiae cells
    Lane 2 : TCA preps from S. cerevisiae cells treated with Hydroxyurea (HU)

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 92 kDa
    Observed band size: 92 kDa
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Western Blot showing ab166859 detecting both unphosphorylated and phosphorylated Rad53.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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