Anti-Involucrin antibody (ab53112)
Key features and details
- Rabbit polyclonal to Involucrin
- Suitable for: ICC/IF, ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Involucrin antibody
See all Involucrin primary antibodies -
Description
Rabbit polyclonal to Involucrin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic peptide derived from human Involucrin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53112 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab53112) This image is courtesy of an Abreview submitted by Carl Hobbs
ab53112 staining human skin sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab53112 at 1/150 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
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Western blot - Anti-Involucrin antibody (ab53112)All lanes : Anti-Involucrin antibody (ab53112) at 1/300 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab53112)ab53112 at 1/50 dilution staining Involucrin in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
