Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP87] to KRT6C - BSA and Azide free
  • Suitable for: ICC, IHC-Fr, Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KRT6C antibody [SP87] - BSA and Azide free
    See all KRT6C primary antibodies

  • Description

    Rabbit monoclonal [SP87] to KRT6C - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 whole cell lysate. IHC-P: Human prostate carcinoma, breast, stomach, prostate, lung squamous cell carcinoma, rectal adenocarcinoma, bladder, bladder transitional cell carcinoma, colon adenocarcinoma, and placenta tissue, rat and mouse skin Flow Cyt: A431 and HaCaT cells. ICC/IF: HaCaT cells. IHC-Fr: Rat and mouse skin tissue.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab240985 is the carrier-free version of ab227643. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240985 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    This data was developed using the same antibody clone in a different buffer formulation (ab227643).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human prostate carcinoma, performed on a Leica Biosystems BOND™ RX instrument. 
    The section was incubated with ab227643 for 10 mins at room temperature.

  • Immunocytochemistry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunocytochemistry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling KRT6C with purified ab227643 at 1/50 (1.92 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

  • Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Flow cytometry analysis of HaCaT (human skin keratinocyte) labeling KRT6C with purified ab227643 at 1/200 dilution (0.48 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    This data was developed using the same antibody clone in a different buffer formulation (ab227643).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat skin, performed on a Leica Biosystems BOND™ RX instrument. 
    The section was incubated with ab227643 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    This data was developed using the same antibody clone in a different buffer formulation (ab227643).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the mouse skin, performed on a Leica Biosystems BOND™ RX instrument. 
    The section was incubated with ab227643 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

  • Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Frozen sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

  • Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Flow Cytometry - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling KRT6C with ab227643 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human breast tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human rectal adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227643)
  • Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)
    Anti-KRT6C antibody [SP87] - BSA and Azide free (ab240985)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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