Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16784] to CD16 - Low endotoxin, Azide free
  • Suitable for: WB, Mass Cytometry, IHC-P
  • Reacts with: Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free
    See all CD16 primary antibodies

  • Description

    Rabbit monoclonal [EPR16784] to CD16 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Mass Cytometry, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver and thymus, Rat liver tissues. IMC: Human tonsil tissue

  • General notes

    ab215977 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Mass Cytometry - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
    Mass Cytometry - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977) This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

    Imaging Mass Cytometry™ (IMC™) image of human tonsil tissue stained with Anti-CD16 antibody [EPR16784]. ab215977 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

    Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of rat liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Using PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198507).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on macrophage of Human thymus tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Using PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198507).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD16 with ab198507 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kupffer cell of Human liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Using PBS instead of primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198507).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)
    Anti-CD16 antibody [EPR16784] - Low endotoxin, Azide free (ab215977)

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