Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TRPV4 antibody [EPR22622-54] (ab231772)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22622-54] to TRPV4
Suitable for: IHC-P
Reacts with: Mouse, Rat

Product name

Anti-TRPV4 antibody [EPR22622-54]
See all TRPV4 primary antibodies
Description

Rabbit monoclonal [EPR22622-54] to TRPV4
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse cerebrum and kidney tissue. Rat kidney and spleen tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22622-54
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (ab231772)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on choroid plexus of mouse cerebrum (PMID: 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (ab231772)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse kidney (PMID:16627472, 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (ab231772)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on rat kidney (PMID:16627472, 18499744) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV4 antibody [EPR22622-54] (ab231772)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling TRPV4 with ab231772 at 1/100 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on blood vessels of rat spleen (PMID:16627472) is observed. The section was incubated with ab231772 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-TRPV4 antibody [EPR22622-54] (ab231772)

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