Anti-DDR2 antibody (ab76967)
Key features and details
- Rabbit polyclonal to DDR2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DDR2 antibody
See all DDR2 primary antibodies -
Description
Rabbit polyclonal to DDR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human DDR2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab86427) -
Positive control
- This antibody gave a positive signal in the following Lysates: Mouse Heart and Rat Heart Tissue in Western Blot and FFPE human heart and skin tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). Abcam recommends using 3% milk as the blocking agent.IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). Abcam recommends using 3% milk as the blocking agent.IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Target
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Function
This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. -
Tissue specificity
The major 10 kDa transcript is expressed in high levels in heart and lung, less in brain, placenta, liver, skeletal muscle, pancreas, and kidney. -
Involvement in disease
Defects in DDR2 are the cause of spondyloepimetaphyseal dysplasia short limb-hand type (SEMD-SL) [MIM:271665]. A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4921 Human
- Entrez Gene: 18214 Mouse
- Entrez Gene: 685781 Rat
- Entrez Gene: 720124 Rhesus monkey
- Omim: 191311 Human
- SwissProt: Q16832 Human
- SwissProt: Q62371 Mouse
- Unigene: 275757 Human
see all -
Alternative names
- CD167 antigen-like family member B antibody
- CD167b antibody
- CD167b antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (ab76967)IHC image of DDR2 staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76967, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-DDR2 antibody (ab76967)All lanes : ab76967 at 1 mg/ml
Lane 1 : Mouse Heart Tissue Lysate
Lane 2 : Rat Heart Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab76967 has been referenced in 6 publications.
- Dowling AR et al. Modulation of interleukin-6 and its effect on late vein wall injury in a stasis mouse model of deep vein thrombosis. JVS Vasc Sci 3:246-255 (2022). PubMed: 35647566
- Li W et al. CGRP derived from cardiac fibroblasts is an endogenous suppressor of cardiac fibrosis. Cardiovasc Res 116:1335-1348 (2020). PubMed: 31504241
- Kazakevych J et al. Transcriptome analysis identifies a robust gene expression program in the mouse intestinal epithelium on aging. Sci Rep 9:10410 (2019). PubMed: 31320724
- Mayfield AE et al. Interleukin-6 Mediates Post-Infarct Repair by Cardiac Explant-Derived Stem Cells. Theranostics 7:4850-4861 (2017). PubMed: 29187908
- Badhwar A et al. The proteome of mouse cerebral arteries. J Cereb Blood Flow Metab 34:1033-46 (2014). Mouse . PubMed: 24667914
- Afonso PV et al. Discoidin domain receptor 2 regulates neutrophil chemotaxis in 3D collagen matrices. Blood 121:1644-50 (2013). Human . PubMed: 23233663
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (ab76967)IHC image of DDR2 staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76967, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-DDR2 antibody (ab76967)All lanes : ab76967 at 1 mg/ml
Lane 1 : Mouse Heart Tissue Lysate
Lane 2 : Rat Heart Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
