Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDR2 antibody (ab76967)

Key features and details

  • Rabbit polyclonal to DDR2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-DDR2 antibody
    See all DDR2 primary antibodies

  • Description

    Rabbit polyclonal to DDR2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Chimpanzee, Rhesus monkey

  • Immunogen

    Synthetic peptide corresponding to Human DDR2 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab86427)

  • Positive control

    • This antibody gave a positive signal in the following Lysates: Mouse Heart and Rat Heart Tissue in Western Blot and FFPE human heart and skin tissue sections.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). Abcam recommends using 3% milk as the blocking agent.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). Abcam recommends using 3% milk as the blocking agent.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing.

  • Tissue specificity

    The major 10 kDa transcript is expressed in high levels in heart and lung, less in brain, placenta, liver, skeletal muscle, pancreas, and kidney.

  • Involvement in disease

    Defects in DDR2 are the cause of spondyloepimetaphyseal dysplasia short limb-hand type (SEMD-SL) [MIM:271665]. A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 1 F5/8 type C domain.
    Contains 1 protein kinase domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD167 antigen-like family member B antibody
    • CD167b antibody
    • CD167b antigen antibody
    • Cell migration inducing protein 20 antibody
    • DDR 2 antibody
    • DDR2 antibody
    • DDR2_HUMAN antibody
    • Discoidin domain containing receptor 2 antibody
    • Discoidin domain receptor 2 antibody
    • Discoidin domain receptor family member 2 antibody
    • discoidin domain receptor tyrosine kinase 2 antibody
    • Discoidin domain-containing receptor 2 antibody
    • discoidin domain-containing receptor tyrosine kinase 2 antibody
    • Hydroxyaryl protein kinase antibody
    • MIG20a antibody
    • Migration inducing gene 16 protein antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase receptor related 3 antibody
    • NTRKR 3 antibody
    • NTRKR3 antibody
    • Receptor protein tyrosine kinase TKT antibody
    • Receptor protein-tyrosine kinase TKT antibody
    • Receptor related 3 antibody
    • receptor-related 3 antibody
    • TKT antibody
    • TYRO 10 antibody
    • TYRO10 antibody
    • Tyrosine kinase receptor related to neurotrophic TRK antibody
    • Tyrosine protein kinase TYRO 10 antibody
    • Tyrosine protein kinase TYRO10 antibody
    • Tyrosine-protein kinase TYRO10 antibody
    • Tyrosylprotein kinase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (ab76967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (ab76967)
    IHC image of DDR2 staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76967, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-DDR2 antibody (ab76967)
    Western blot - Anti-DDR2 antibody (ab76967)
    All lanes : ab76967 at 1 mg/ml

    Lane 1 : Mouse Heart Tissue Lysate
    Lane 2 : Rat Heart Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?
    Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References (6)

Publishing research using ab76967? Please let us know so that we can cite the reference in this datasheet.

ab76967 has been referenced in 6 publications.

  • Dowling AR  et al. Modulation of interleukin-6 and its effect on late vein wall injury in a stasis mouse model of deep vein thrombosis. JVS Vasc Sci 3:246-255 (2022). PubMed: 35647566
  • Li W  et al. CGRP derived from cardiac fibroblasts is an endogenous suppressor of cardiac fibrosis. Cardiovasc Res 116:1335-1348 (2020). PubMed: 31504241
  • Kazakevych J  et al. Transcriptome analysis identifies a robust gene expression program in the mouse intestinal epithelium on aging. Sci Rep 9:10410 (2019). PubMed: 31320724
  • Mayfield AE  et al. Interleukin-6 Mediates Post-Infarct Repair by Cardiac Explant-Derived Stem Cells. Theranostics 7:4850-4861 (2017). PubMed: 29187908
  • Badhwar A  et al. The proteome of mouse cerebral arteries. J Cereb Blood Flow Metab 34:1033-46 (2014). Mouse . PubMed: 24667914
  • Afonso PV  et al. Discoidin domain receptor 2 regulates neutrophil chemotaxis in 3D collagen matrices. Blood 121:1644-50 (2013). Human . PubMed: 23233663

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (ab76967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (ab76967)
    IHC image of DDR2 staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76967, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-DDR2 antibody (ab76967)
    Western blot - Anti-DDR2 antibody (ab76967)
    All lanes : ab76967 at 1 mg/ml

    Lane 1 : Mouse Heart Tissue Lysate
    Lane 2 : Rat Heart Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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