Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759)
Key features and details
- Rabbit polyclonal to SARS-CoV-2 Spike Glycoprotein S1
- Suitable for: IHC-P, ICC, ELISA, WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Rabbit polyclonal to SARS-CoV-2 Spike Glycoprotein S1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, ELISA, WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Human coronavirus
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Immunogen
Synthetic peptide corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. Peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) Spike S1 glycoprotein. The immunogen is located within the first 50 amino acids. Genbank: QHD43416
Database link: P0DTC2 -
Positive control
- WB: SARS-Cov2 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate; SARS COV-2 Spike glycoprotein transfected HEK-293 whole cell lysate; Recombinant SARS COV-2 Spike Glycoprotein S1 IHC-P: Human lung tissue from SARS COV-2 patient. ICC: SARS COV-2 Spike Glycoprotein transfected HEK-293 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) Courtesy of Hallgeir Rui
Formaldehyde-fixed, paraffin-embedded human lung tissue from a COVID-19 patient was stained for SARS-CoV-2 Spike Glycoprotein S1 using ab275759 at 1 μg/mL, followed by a Goat Anti-Rabbit IgG conjugate at 1/250 dilution. Counter stained with hematoxylin. Primary incubation overnight at 4°C.
Heat-mediated antigen retrieval with citrate buffer, pH 6.0. Tissue was blocked with 10% serum for 1 hour at room temperature.
40X magnification.
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Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759)Immunofluorescent analysis of 4% paraformaldehyde-fixed Spike transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells labelling SARS-CoV-2 Spike Glycoprotein S1 with ab275759 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (Green). The nuclear counter stain was DAPI (Blue).
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Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759)All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) at 1 µg/ml
Lane 1 : SARS-Cov1 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lane 2 : SARS-Cov2 spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lane 3 : MERS Spike protein (DDDDK-tagged) transfected HEK-293 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Observed band size: 200 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Green - ab275759 observed at 200kDa.
ab275759 was shown to react with SARS-CoV-2 Spike Glycoprotein S1 in western blot. Membranes were blocked in 3% milk before incubation with ab275759 overnight at 4°C at 1 µg/ml. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) secondary antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) Courtesy of Hallgeir RuiAll lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) at 1 µg/ml
Lane 1 : Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : SARS-COV-2 Spike over-expressing HEK-293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incubation for 1 hour at room temperature.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) Courtesy of Hallgeir RuiFormaldehyde-fixed, paraffin-embedded human lung tissue from a COVID-19 patient was stained for SARS-CoV-2 Spike Glycoprotein S1 using ab275759 at 1 μg/mL, followed by a Goat Anti-Rabbit IgG conjugate at 1/250 dilution. Counter stained with hematoxylin. Primary incubation overnight at 4°C.
Heat-mediated antigen retrieval with citrate buffer, pH 6.0. Tissue was blocked with 10% serum for 1 hour at room temperature.
40X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) Courtesy of Hallgeir RuiFormaldehyde-fixed, paraffin-embedded human lung tissue from a COVID-19 patient was stained for SARS-CoV-2 Spike Glycoprotein S1 using ab275759 at 1 μg/mL, followed by a Goat Anti-Rabbit IgG conjugate at 1/250 dilution. Counter stained with hematoxylin. Primary incubation overnight at 4°C.
Heat-mediated antigen retrieval with citrate buffer, pH 6.0. Tissue was blocked with 10% serum for 1 hour at room temperature.
40X magnification.
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Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759)Lane 1 : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) at 1 µg/ml
Lane 2 : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) at 2 µg/ml
All lanes : Recombinant SARS-COV-2 Spike Glycoprotein S1 (aa 16 - 685), 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incubation for 1 hour at room temperature.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) Courtesy of Hallgeir RuiFormaldehyde-fixed, paraffin-embedded human lung tissue from a COVID-19 patient was stained for SARS-CoV-2 Spike Glycoprotein S1 using ab275759 at 1 μg/mL, followed by a Goat Anti-Rabbit IgG conjugate at 1/250 dilution. Counter stained with hematoxylin. Primary incubation overnight at 4°C.
Heat-mediated antigen retrieval with citrate buffer, pH 6.0. Tissue was blocked with 10% serum for 1 hour at room temperature.
40X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) Courtesy of Hallgeir RuiFormaldehyde-fixed, paraffin-embedded human lung tissue from a COVID-19 patient was stained for SARS-CoV-2 Spike Glycoprotein S1 using ab275759 at 1 μg/mL, followed by a Goat Anti-Rabbit IgG conjugate at 1/250 dilution. Counter stained with hematoxylin. Primary incubation overnight at 4°C.
Heat-mediated antigen retrieval with citrate buffer, pH 6.0. Tissue was blocked with 10% serum for 1 hour at room temperature.
40X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759) Courtesy of Hallgeir RuiFormaldehyde-fixed, paraffin-embedded human lung tissue from a COVID-19 patient was stained for SARS-CoV-2 Spike Glycoprotein S1 using ab275759 at 1 μg/mL, followed by a Goat Anti-Rabbit IgG conjugate at 1/250 dilution. Counter stained with hematoxylin. Primary incubation overnight at 4°C.
Heat-mediated antigen retrieval with citrate buffer, pH 6.0. Tissue was blocked with 10% serum for 1 hour at room temperature.
40X magnification.
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ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (ab275759)A direct ELISA was performed using a recombinant SARS-COV-2 Spike 1 Glycoprotein (aa 16 - 685) as the coating antigen and ab275759 as capture antibody. A goat anti-rabbit IgG-HRP conjugate was used as the secondary antibody at a 1/20000 dilution.