Anti-Ceramide glucosyltransferase antibody (ab124296)
Key features and details
- Rabbit polyclonal to Ceramide glucosyltransferase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Ceramide glucosyltransferase antibody
See all Ceramide glucosyltransferase primary antibodies -
Description
Rabbit polyclonal to Ceramide glucosyltransferase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Ceramide glucosyltransferase aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab140400) -
Positive control
- This antibody gave a positive signal in MCF7 and A375 whole cell lysates, and in the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Spleen; Rat Spleen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa). Target
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Function
Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase". -
Tissue specificity
Found in all tissues examined. -
Pathway
Lipid metabolism; sphingolipid metabolism. -
Sequence similarities
Belongs to the glycosyltransferase 2 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 7357 Human
- Entrez Gene: 22234 Mouse
- Entrez Gene: 83626 Rat
- Omim: 602874 Human
- SwissProt: Q16739 Human
- SwissProt: O88693 Mouse
- SwissProt: Q9R0E0 Rat
- Unigene: 304249 Human
see all -
Alternative names
- CEGT_HUMAN antibody
- Ceramide glucosyltransferase antibody
- GCS antibody
see all
Images
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Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296)All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 : Spleen (Rat) Tissue Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa, 36 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124296 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (2)
ab124296 has been referenced in 2 publications.
- Hayashi Y et al. Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels. J Biol Chem 293:17505-17522 (2018). PubMed: 30242129
- Sun R et al. Lowered humidity produces human epidermal equivalents with enhanced barrier properties. Tissue Eng Part C Methods 21:15-22 (2015). PubMed: 24803151
Images
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Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296)All lanes : Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 : Spleen (Rat) Tissue Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : A375 (Human melanoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa, 36 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124296 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
