Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IFNL4 antibody [51-1] (ab196984)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [51-1] to IFNL4
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-IFNL4 antibody [51-1]
    See all IFNL4 primary antibodies

  • Description

    Rabbit monoclonal [51-1] to IFNL4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    BL
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 transfected with IFNL4 with with Halo-tag, whole cell lysate

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    This product has switched from a hybridoma to recombinant production method on 06th January 2021

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    51-1

Images

  • Western blot - Anti-IFNL4 antibody [51-1] (ab196984)
    Western blot - Anti-IFNL4 antibody [51-1] (ab196984)
    All lanes : Anti-IFNL4 antibody [51-1] (ab196984) at 1/1000 dilution

    Lane 1 : Empty vector transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
    Lane 2 : HepG2 transfected with IFNL4 with with Halo-tag, whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed size is larger (50 kDa for IFNL4-Halo than the predicted size because of the Halo-tag.

  • Western blot - Anti-IFNL4 antibody [51-1] (ab196984)
    Western blot - Anti-IFNL4 antibody [51-1] (ab196984) This image is courtesy of Ludmila Prokunina-Olsson
    All lanes : Anti-IFNL4 antibody [51-1] (ab196984) at 1/1500 dilution

    Lane 1 : Recombinant Interferon lambda 4 protein (250 ng)
    Lane 2 : Recombinant Interferon lambda 4 protein (50 ng)

    Predicted band size: 20 kDa



    Prokunina-Olsson, NG, 2013 and Onabajo et al, JICR, 2015

    This image was generated from the hybridoma version of the product.

  • Blocking - Anti-IFNL4 antibody [51-1] (ab196984)
    Blocking - Anti-IFNL4 antibody [51-1] (ab196984) This image is courtesy of Ludmila Prokunina-Olsson

    Stable inducible HepG2-IFN-λ4-ISRE-Luc cells were induced with 0.5 mg/ml doxycycline for 16hours and then treated with PBS (negative control) and rabbit IgG (20µg/ml) for 22 hours and assayed for luciferase expression.  α-IFN-λ4 (ab196984) blocks the ability of IFN-λ4 to induce of ISRE-Luc reporter by 70%.

    This image was generated from the hybridoma version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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