Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [51-1] to IFNL4
Suitable for: WB
Reacts with: Human, Recombinant fragment

Product name

Anti-IFNL4 antibody [51-1]
See all IFNL4 primary antibodies
Description

Rabbit monoclonal [51-1] to IFNL4
Host species

Rabbit
Tested Applications & Species

Application Species
BL
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2 transfected with IFNL4 with with Halo-tag, whole cell lysate
General notes
This antibody clone is manufactured by Abcam.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

This product has switched from a hybridoma to recombinant production method on 06^th January 2021
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
BL	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

51-1

Western blot - Anti-IFNL4 antibody [51-1] (ab196984)

All lanes : Anti-IFNL4 antibody [51-1] (ab196984) at 1/1000 dilution

Lane 1 : Empty vector transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
Lane 2 : HepG2 transfected with IFNL4 with with Halo-tag, whole cell lysate

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 20 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

The observed size is larger (50 kDa for IFNL4-Halo than the predicted size because of the Halo-tag.
Western blot - Anti-IFNL4 antibody [51-1] (ab196984) This image is courtesy of Ludmila Prokunina-Olsson

All lanes : Anti-IFNL4 antibody [51-1] (ab196984) at 1/1500 dilution

Lane 1 : Recombinant Interferon lambda 4 protein (250 ng)
Lane 2 : Recombinant Interferon lambda 4 protein (50 ng)

Predicted band size: 20 kDa

Prokunina-Olsson, NG, 2013 and Onabajo et al, JICR, 2015

This image was generated from the hybridoma version of the product.
Blocking - Anti-IFNL4 antibody [51-1] (ab196984) This image is courtesy of Ludmila Prokunina-Olsson

Stable inducible HepG2-IFN-λ4-ISRE-Luc cells were induced with 0.5 mg/ml doxycycline for 16hours and then treated with PBS (negative control) and rabbit IgG (20µg/ml) for 22 hours and assayed for luciferase expression. α-IFN-λ4 (ab196984) blocks the ability of IFN-λ4 to induce of ISRE-Luc reporter by 70%.

This image was generated from the hybridoma version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com