Anti-beta Tubulin antibody (ab21057)
Key features and details
- Goat polyclonal to beta Tubulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta Tubulin antibody
See all beta Tubulin primary antibodies -
Description
Goat polyclonal to beta Tubulin -
Host species
Goat -
Specificity
ab21057 detects a band of ~50kDa in Hela and A431 lysates (data for A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Monkey, African green monkey
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Immunogen
Synthetic peptide corresponding to Human beta Tubulin aa 1-100.
(Peptide available asab20775)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Tubulin antibody (ab21057)All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate withHuman beta Tubulin peptide (ab20775) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit anti Goat polyclonal at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?ab21057 detects a band of ~ 50kDa in Hela whole cell lysate, HeLa nuclear lysate and A431 lysates (data for HeLa nuclear and A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.
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Western blot - Anti-beta Tubulin antibody (ab21057)Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
20ug of Lysate per lane and detection using ab21057 diluted to 1ug/ml.
Lane 1: Hela cell lysate.
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK293 cell lysate
Lane 5: HepG2 cell lysate.
