Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTG4 antibody [EPRZJU-21] (ab206914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRZJU-21] to BTG4
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-BTG4 antibody [EPRZJU-21]
    See all BTG4 primary antibodies

  • Description

    Rabbit monoclonal [EPRZJU-21] to BTG4

  • Host species

    Rabbit

  • Specificity

    We have not been able to detect the endogenous mouse BTG4 with ab206914.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate transfected with mouse BTG4 with GFP-tag. ICC/IF: HeLa cells transfected with GFP-BTG4. IP: 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPRZJU-21

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
    Western blot - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
    All lanes : Anti-BTG4 antibody [EPRZJU-21] (ab206914) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate lysate transfected with mouse BTG4 with GFP-tag
    Lane 3 : Human ovary lysate
    Lane 4 : Mouse ovary lysate
    Lane 5 : Mouse testis lysate
    Lane 6 : Rat ovary lysate
    Lane 7 : Rat testis lysate
    Lane 8 : Mouse P0 olfactory epithelium lysate
    Lane 9 : Rat P0 olfactory epithelium lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
    Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] (ab206914)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BTG4 with ab206914 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

    Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell transfected with GFP-BTG4.

    The nuclear counterstain is DAPI (blue).

  • Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (ab206914)
    Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (ab206914)

    BTG4 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) transfected with mouse BTG4 with HA-tag whole cell lysate with ab206914 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab206914 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: 293T transfected with mouse BTG4 with HA-tag whole cell lysate, 10µg (Input).

    Lane 2: ab206914 IP in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206914 in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-BTG4 antibody [EPRZJU-21] (ab206914)
    Anti-BTG4 antibody [EPRZJU-21] (ab206914)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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