Anti-Ataxin 1 antibody [N65/37] (ab186393)
![Anti-Ataxin 1 antibody [N65/37] (ab186393)](https://www.abcam.com/ps/products/186/ab186393/Images/ab186393-304406-anti-ataxin-1-antibody-n65-37-immunocytochemistry.jpg "Anti-Ataxin 1 antibody [N65/37] (ab186393)")
Key features and details
- Mouse monoclonal [N65/37] to Ataxin 1
- Suitable for: WB, ICC
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
-
Product name
Anti-Ataxin 1 antibody [N65/37]
See all Ataxin 1 primary antibodies -
Description
Mouse monoclonal [N65/37] to Ataxin 1 -
Host species
Mouse -
Specificity
No cross-reactivity against phosphor S751-Ataxin 1. Minimal cross-reactivity against S751A mutant of Ataxin 1 by ELISA and immunofluorescence and negative by immunoblot. -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Mouse Ataxin 1 aa 700 to the C-terminus (C terminal).
Database link: P54254 -
Positive control
- COS cells transiently transfected with Ataxin 1.
-
General notes
The clone number has been updated from S65-37 to N65/37, both clone numbers name the same antibody clone.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N65/37 -
Isotype
IgG1 -
Research areas
Images
-
Immunocytochemistry - Anti-Ataxin 1 antibody [N65/37] (ab186393)
Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.
-
![Western blot - Anti-Ataxin 1 antibody [N65/37] (ab186393)](https://www.abcam.com/ps/products/186/ab186393/Images/ab186393-304388-anti-ataxin-1-antibody-n65-37-western-blot.jpg)
Western blot - Anti-Ataxin 1 antibody [N65/37] (ab186393)Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S65-37. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~84 kDa.
