Anti-Ataxin 1 antibody [N76/8] (ab186265)
![Anti-Ataxin 1 antibody [N76/8] (ab186265)](https://www.abcam.com/ps/products/186/ab186265/Images/ab186265-312192-anti-ataxin-1-antibody-n76-8-western-blot.jpg "Anti-Ataxin 1 antibody [N76/8] (ab186265)")
Key features and details
- Mouse monoclonal [N76/8] to Ataxin 1
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-Ataxin 1 antibody [N76/8]
See all Ataxin 1 primary antibodies -
Description
Mouse monoclonal [N76/8] to Ataxin 1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Ataxin 1 aa 150-250.
Database link: P45254 -
Positive control
- Rat brain lysate.
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General notes
The clone number has been updated from S76-8 to N76/8, both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N76/8 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Ataxin 1 antibody [N76/8] (ab186265)Anti-Ataxin 1 antibody [N76/8] (ab186265) at 1/200 dilution + Monkey COS-1 cells transfected with Ataxin- 1 at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT
Predicted band size: 84 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 1 antibody [N76/8] (ab186265)](https://www.abcam.com/ps/products/186/ab186265/Images/ab186265-312191-anti-ataxin-1-antibody-n76-8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 1 antibody [N76/8] (ab186265)Immunocytochemistry/Immunofluorescence analysis of 4% Formaldehyde-fixed human SK-N-BE Cells (Human Neuroblastoma cells) labeling Ataxin 1 (green) using ab186265 at 1/100 dilution P for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.
