ab63337 at 1/1000 dilution staining DDR2 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections).

ab63337 at 1/1000 dilution staining DDR2 in human A549 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® conjugated Goat anti-mouse was used as secondary. The actin filaments were labeled red with DY-554 phalloidin whilst nuclei were stained blue with DRAQ5 fluorescent DNA dye.

Overlay histogram showing A549 cells stained with ab63337 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63337, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a (ab170191, 0.5μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.