Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)
Key features and details
- Rabbit polyclonal to 12 Lipoxygenase/ALOX12 - C-terminal
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal
See all 12 Lipoxygenase/ALOX12 primary antibodies -
Description
Rabbit polyclonal to 12 Lipoxygenase/ALOX12 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human 12 Lipoxygenase/ALOX12 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. NCBI Accession No. NP_000688.2.
Database link: P18054 -
Positive control
- K562 cell lysate; Human heart tisue; A549 and K562 cells.
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General notes
This product was previously labelled as 12 Lipoxygenase
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506) at 1/1000 dilution + K562 cell lysate at 35 µg
Predicted band size: 76 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling 12 Lipoxygenase/ALOX12 using ab211506 at a 1/50 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)Immunofluorescence analysis of A549 cells, labeling 12 Lipoxygenase/ALOX12 using ab211506 at a 1/100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1/400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
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Flow Cytometry - Anti-12 Lipoxygenase/ALOX12 antibody - C-terminal (ab211506)Flow Cytometry analysis of K562 cells labeling 12 Lipoxygenase/ALOX12 (bottom histogram), using ab211506 at a 1/10 dilution, and negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
