Anti-liver FABP antibody (ab76812)
Key features and details
- Rabbit polyclonal to liver FABP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-liver FABP antibody
See all liver FABP primary antibodies -
Description
Rabbit polyclonal to liver FABP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig, Orangutan
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Immunogen
Synthetic peptide corresponding to Human liver FABP aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab91438) -
Positive control
- This antibody gave a positive signal in WB on human liver tissue lysate. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab76812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). Target
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Function
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2168 Human
- Entrez Gene: 14080 Mouse
- Entrez Gene: 24360 Rat
- Omim: 134650 Human
- SwissProt: P07148 Human
- SwissProt: P12710 Mouse
- SwissProt: P02692 Rat
- Unigene: 380135 Human
see all -
Alternative names
- FABP 1 antibody
- FABP1 antibody
- FABPL antibody
see all
Images
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Western blot - Anti-liver FABP antibody (ab76812)Anti-liver FABP antibody (ab76812) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 160 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody (ab76812)ICC/IF image of ab76812 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76812, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-liver FABP antibody (ab76812)Anti-liver FABP antibody (ab76812) at 1 µg/ml + Recombinant Human liver FABP protein (ab82994) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Exposure time: 8 minutes
Protocols
Datasheets and documents
References (0)
ab76812 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-liver FABP antibody (ab76812)Anti-liver FABP antibody (ab76812) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 160 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody (ab76812)
ICC/IF image of ab76812 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76812, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-liver FABP antibody (ab76812)Anti-liver FABP antibody (ab76812) at 1 µg/ml + Recombinant Human liver FABP protein (ab82994) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Exposure time: 8 minutes
