Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PHGDH/Malate dehydrogenase protein
See all PHGDH/Malate dehydrogenase proteins and peptides -
Biological activity
Specific Activity: ≥80 pmol/min/µg
Assay Conditions: The reaction mixture contained 100 mM Tris-HCl, pH 8.8, 400 mM NaCl2, 0.2 mM DTT, 2.5 mM 3-phospho-D-glyceric acid, 1 mM NAD, and various amount of ab198455 in a final volume of 50 µL. Reactions were initiated with the addition of ab198455, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature. -
Purity
> 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEG LIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPN GNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGI LGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLC DFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQ SGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIA VQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSP KGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEA GLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNG AVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLV SDGETWHVMGISSLLPSLEAWKQHVTEAFQFHFHHHHHH -
Predicted molecular weight
58 kDa including tags -
Amino acids
1 to 533 -
Tags
His tag C-Terminus -
Additional sequence information
GenBank Accession No. NM_006623
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.16% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)
Specific activity of ab198455.
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SDS-PAGE - Recombinant human PHGDH/Malate dehydrogenase protein (ab198455)4-20% SDS-PAGE analysis of ab198455 with Coomassie staining.
Lane 1: 5.5 µg ab198455
Lane 2: Protein marker
