Human Cathepsin B ELISA Kit (ab272205)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 23.44 pg/ml
- Range: 109.38 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Cathepsin B ELISA Kit
See all Cathepsin B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.8% Inter-assay Sample n Mean SD CV% Serum 3 6% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
23.44 pg/ml -
Range
109.38 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 87% - 106% Saliva 108 95% - 108% Urine 91 84% - 100% Serum 98 95% - 99% Hep Plasma 103 92% - 113% EDTA Plasma 114 110% - 122% Cit plasma 93 88% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cathepsin B ELISA kit (ab272205) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin B protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cathepsin B with 41.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Cathepsin B is a part of a family of cysteine lysosomal proteases. Cathepsin B is synthesized as a preproenzyme in the endoplasmic reticulum and activated after transportation to the Golgi apparatus. Cathepsin B is overexpressed in invasive and metastatic cancers and is such a potential biomarker for a variety of cancers. Double knockout of cathepsin B and cathepsin L in mice is lethal due to their role in the central nervous system.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cathepsin B Capture Antibody 1 x 600µl 10X Human Cathepsin B Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Cathepsin B Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Alternative names
- Amyloid precursor protein secretase
- APP secretase
- APPS
see all -
Database links
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Human Cathespin B competitor curve comparisonStandard Curve comparison between Human Cathepsin B SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
-
Example of human Cathepsin B standard curve in Sample Diluent NS.The Cathepsin B standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Cathepsin B in human serum and plasma samples.The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 8%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 54.38 ng/mL in serum, 56.17 ng/mL in plasma (citrate), 55.98 ng/ml in plasma (heparin), 55.79 ng/ml in plasma (EDTA).
-
Interpolated concentrations of native Cathepsin B in treated human PBMC cell culture supernatant and male saliva samples.PBMC supernatant samples were treated with 1.5% PHA-M for 46 hours. The concentrations of Cathepsin B were measured in duplicates, interpolated from the Cathepsin B standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 4% and saliva 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 103.07 ng/mL in treated PBMC supernatant and 7.00 ng/mL in male saliva.
-
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cathepsin B concentration was determined to be 98.94 ng/mL with a range of 54.68 – 219.68 ng/mL.
-
Sandwich ELISA - Human Cathepsin B ELISA Kit (ab272205)To learn more about the advantages of recombinant antibodies see here.
