Human NrCAM ELISA Kit (ab267568)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.328 ng/ml - 80 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NrCAM ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3 ng/ml -
Range
0.328 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108 68% - 143% Plasma 79 68% - 106% Cell culture media 115 99% - 133% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human NrCAM ELISA Kit is designed for the quantitative determination of NRCAM in cell culture supernatants, plasma and serum samples.
ΔNote: Human NrCAM concentration is low in normal serum and plasma samples and may not be detectable in this assay.
This assay employs an antibody specific for human NrCAM coated on a 96-well plate. Standards and samples are pipetted into the wells and NrCAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human NrCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NrCAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human NrCAM coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Human NrCAM Detection Antibody 2 vials Human NrCAM Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier. -
Tissue specificity
Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Bravo
- hBravo
- Neuronal cell adhesion molecule
see all -
Database links
- Entrez Gene: 4897 Human
- Omim: 601581 Human
- SwissProt: Q92823 Human
- Unigene: 21422 Human
Images
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Standard curve
Human NrCAM ELISA kit (ab267568) Standard curves in Assay Diluent A and Assay Diluent D.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.