Anti-Syndecan-1 antibody (ab60199)
Key features and details
- Rabbit polyclonal to Syndecan-1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Syndecan-1 antibody
See all Syndecan-1 primary antibodies -
Description
Rabbit polyclonal to Syndecan-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide (Human) surrounding amino acid 298
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Positive control
- Mouse tissue lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Syndecan-1 antibody (ab60199)Anti-Syndecan-1 antibody (ab60199) at 4 µg/ml + Mouse tissue lysate
Predicted band size: 34 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
The observed band size is larger than predicted due to haparan sulfate and chondroitin sulfate modifications. -
Western blot - Anti-Syndecan-1 antibody (ab60199) Image from Taylor DR et al, PLoS Pathog. 2009 Nov;5(11):e1000666. Epub 2009 Nov 20, Fig 6.Anti-Syndecan-1 antibody (ab60199) at 1/2000 dilution + lysate prepared from ScN2a cells.
Secondary
peroxidase-conjugated secondary
Developed using the ECL technique.
Predicted band size: 34 kDa
Samples were prepared in dissociation buffer and boiled for 5 minutes. Proteins were resolved by SDS-PAGE using either 7–17% polyacrylamide gradient gels or 14.5% polyacrylamide gels. For western blot analysis, resolved proteins were transferred to Immobilon P polyvinylidene difluoride membrane. The membrane was blocked by incubation for 1 hour with phosphate-buffered saline containing 0.1% (v/v) Tween-20 and 5% (w/v) dried milk powder. Antibody incubations were performed in phosphate-buffered saline-Tween containing 2% (v/v) bovine serum albumin. Horseradish peroxidase-conjugated streptavidin was used at 1/2000.
