Anti-NCAM2 antibody (ab130742)
Key features and details
- Rabbit polyclonal to NCAM2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NCAM2 antibody
See all NCAM2 primary antibodies -
Description
Rabbit polyclonal to NCAM2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human NCAM2 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab130815) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Brain; Mouse Brain; Mouse Olfactory Bulb; Rat Olfactory Bulb.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanAll applications RabbitHorseChimpanzeeGorillaChinese hamsterOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa).Target
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Function
May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons. -
Tissue specificity
Expressed most strongly in adult and fetal brain. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4685 Human
- Entrez Gene: 17968 Mouse
- Entrez Gene: 288280 Rat
- Omim: 602040 Human
- SwissProt: O15394 Human
- SwissProt: O35136 Mouse
- Unigene: 473450 Human
- Unigene: 433941 Mouse
see all -
Alternative names
- Cell adhesion molecule, neural, 2 antibody
- MGC51008 antibody
- N CAM 2 antibody
see all
Images
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Western blot - Anti-NCAM2 antibody (ab130742)All lanes : Anti-NCAM2 antibody (ab130742) at 1 µg/ml
Lanes 1 & 5 : Human brain tissue lysate - total protein (ab29466)
Lanes 2 & 6 : Brain (Mouse) Tissue Lysate
Lanes 3 & 7 : Mouse Olfactory Bulb Lysate
Lanes 4 & 8 : Rat Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
NCAM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab130742 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-NCAM2 antibody (ab130742)All lanes : Anti-NCAM2 antibody (ab130742) at 1 µg/ml
Lanes 1 & 5 : Human brain tissue lysate - total protein (ab29466)
Lanes 2 & 6 : Brain (Mouse) Tissue Lysate
Lanes 3 & 7 : Mouse Olfactory Bulb Lysate
Lanes 4 & 8 : Rat Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
NCAM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
