Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µg 278 083 ₸

Order now and get it on Thursday March 04, 2021

Anti-Syndecan-1 antibody [1A3H4] (ab181789)

Key features and details

Mouse monoclonal [1A3H4] to Syndecan-1
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Human
Isotype: IgG1

Product name

Anti-Syndecan-1 antibody [1A3H4]
See all Syndecan-1 primary antibodies
Description

Mouse monoclonal [1A3H4] to Syndecan-1
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human Syndecan-1 aa 28-171 (internal sequence). Expressed in E. Coli.
Sequence:
NLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTS PEPTGLEATA ASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRP RETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPS

Database link: P18827
Run BLAST with Run BLAST with
Positive control
- Human ovarian and rectum cancer tissues; HepG2 cells; MCF7, HeLa, HepG2, T47D, SW620, Jurkat and mouse NIH 3T3 cell lysates; Human Syndecan-1 recombinant protein (amino acids 28-171); HEK293 cell lysate transfected with Syndecan-1 (amino acids 28-171)-hIgGFc.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR214200-21 are from Tissue Culture Supernatant

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

1A3H4
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [1A3H4] (ab181789)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Syndecan-1 with ab181789 at 1/200 dilution followed by DAB staining.
Western blot - Anti-Syndecan-1 antibody [1A3H4] (ab181789)

Anti-Syndecan-1 antibody [1A3H4] (ab181789) at 1/500 dilution + Human Syndecan-1 recombinant protein (amino acids 28-171)

Predicted band size: 32 kDa
Western blot - Anti-Syndecan-1 antibody [1A3H4] (ab181789)

All lanes : Anti-Syndecan-1 antibody [1A3H4] (ab181789) at 1/500 dilution

Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with Syndecan-1 (amino acids 28-171)-hIgGFc

Predicted band size: 32 kDa
Western blot - Anti-Syndecan-1 antibody [1A3H4] (ab181789)

All lanes : Anti-Syndecan-1 antibody [1A3H4] (ab181789) at 1/500 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : T47D cell lysate
Lane 5 : SW620 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : mouse NIH 3T3 cell lysate

Predicted band size: 32 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [1A3H4] (ab181789)

Immunofluorescent analysis of HepG2 cells labeling Syndecan-1 with ab181789 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry - Anti-Syndecan-1 antibody [1A3H4] (ab181789)

Flow cytometric analysis of HepG2 cells labeling Syndecan-1 with ab181789 at 1/200 dilution (green); negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [1A3H4] (ab181789)

Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Syndecan-1 with ab181789 at 1/200 dilution followed by DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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