Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Stomatin antibody [EPR10420] (ab169524)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10420] to Stomatin
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-Stomatin antibody [EPR10420]
See all Stomatin primary antibodies
Description

Rabbit monoclonal [EPR10420] to Stomatin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Stomatin.
Database link: P27105
Positive control
- Human fetal heart, HeLa and HepG2 lysates; HeLa cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Previously labelled as STOM.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10420
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stomatin antibody [EPR10420] (ab169524)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Cytoplasmic staining on human ovarian carcinoma. The section was incubated with ab169523 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)

All lanes : Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : HepG2 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa
Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)

Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate

Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 32 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (ab169524)

Immunofluorescent analysis of HeLa cells labeling Stomatin with ab169524 at 1/100 dilution. DAPI nuclear staining (blue).
Flow Cytometry - Anti-Stomatin antibody [EPR10420] (ab169524)

Flow cytometric analysis of permeabilized HeLa cells labeling Stomatin with ab169524 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Anti-Stomatin antibody [EPR10420] (ab169524)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com