Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Stomatin antibody [EPR10420] (ab169524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10420] to Stomatin
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Stomatin antibody [EPR10420]
    See all Stomatin primary antibodies

  • Description

    Rabbit monoclonal [EPR10420] to Stomatin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Stomatin.
    Database link: P27105

  • Positive control

    • Human fetal heart, HeLa and HepG2 lysates; HeLa cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as STOM.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10420

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stomatin antibody [EPR10420] (ab169524)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stomatin antibody [EPR10420] (ab169524)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ADRA1B with ab169523 at 1/500 (1.242 ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Cytoplasmic staining on human ovarian carcinoma. The section was incubated with ab169523 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)
    Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)
    All lanes : Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 32 kDa

  • Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)
    Western blot - Anti-Stomatin antibody [EPR10420] (ab169524)
    Anti-Stomatin antibody [EPR10420] (ab169524) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (ab169524)
    Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10420] (ab169524)

    Immunofluorescent analysis of HeLa cells labeling Stomatin with ab169524 at 1/100 dilution. DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-Stomatin antibody [EPR10420] (ab169524)
    Flow Cytometry - Anti-Stomatin antibody [EPR10420] (ab169524)

    Flow cytometric analysis of permeabilized HeLa cells labeling Stomatin with ab169524 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Anti-Stomatin antibody [EPR10420] (ab169524)
    Anti-Stomatin antibody [EPR10420] (ab169524)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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