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Anti-Stomatin antibody [EPR10421] (ab166623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10421] to Stomatin
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Stomatin antibody [EPR10421]
    See all Stomatin primary antibodies

  • Description

    Rabbit monoclonal [EPR10421] to Stomatin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Stomatin.
    Database link: P27105

  • Positive control

    • Human fetal heart, HeLa, HepG2, Neuro-2a and Raw264.7 lysates, HeLa cells, permeabilized Neuro-2a cells, immunoprecipitation pellet from fetal heart lysate

  • General notes

    Previously labelled as STOM.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10421

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Stomatin antibody [EPR10421] (ab166623)
    Western blot - Anti-Stomatin antibody [EPR10421] (ab166623)
    All lanes : Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : HeLa lysates
    Lane 3 : HepG2 lysates
    Lane 4 : Neuro-2a lysates
    Lane 5 : Raw264.7 lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] (ab166623)
    Immunocytochemistry/ Immunofluorescence - Anti-Stomatin antibody [EPR10421] (ab166623)

    Immunofluorescence analysis of HeLa cells labeling Stomatin with ab166623 at 1/100 dilution.

  • Flow Cytometry - Anti-Stomatin antibody [EPR10421] (ab166623)
    Flow Cytometry - Anti-Stomatin antibody [EPR10421] (ab166623)
    Flow cytometric analysis of permeabilized Neuro-2a cells using ab166623 (red) at 1/10 dilution or a rabbit IgG (negative) (green).
  • Immunoprecipitation - Anti-Stomatin antibody [EPR10421] (ab166623)
    Immunoprecipitation - Anti-Stomatin antibody [EPR10421] (ab166623)

    Immunoprecipitation of Stomatin in pellet from Human fetal heart lysate using ab166623 at 1/10 dilution, followed by WB analysis of Stomatin.



    Anti-Stomatin antibody [EPR10421] (ab166623) at 1/1000 dilution + immunoprecipitation pellet from Human fetal heart lysate at 10 µg
  • Anti-Stomatin antibody [EPR10421] (ab166623)
    Anti-Stomatin antibody [EPR10421] (ab166623)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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