Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP22 antibody [EPR4352(2)] (ab109435)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4352(2)] to USP22
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-USP22 antibody [EPR4352(2)]
    See all USP22 primary antibodies

  • Description

    Rabbit monoclonal [EPR4352(2)] to USP22

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide corresponding to residues in human USP22

  • Positive control

    • HeLa, HAP1, human placenta and HT-1376 lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-USP22 antibody [EPR4352(2)] (ab109435)
    Western blot - Anti-USP22 antibody [EPR4352(2)] (ab109435)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: USP22 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109435 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.


    Ab109435 was shown to specifically react with USP22 in wild-type cells along with additional cross-reactive bands. The band was not seen in USP22 knockout HAP1 cells. Wild-type and USP22 knockout samples were subjected to SDS-PAGE. ab109435 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-USP22 antibody [EPR4352(2)] (ab109435)
    Western blot - Anti-USP22 antibody [EPR4352(2)] (ab109435)
    All lanes : Anti-USP22 antibody [EPR4352(2)] (ab109435) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Human placenta lysate
    Lane 3 : HT-1376 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 60 kDa

  • Flow Cytometry - Anti-USP22 antibody [EPR4352(2)] (ab109435)
    Flow Cytometry - Anti-USP22 antibody [EPR4352(2)] (ab109435)
    Flow cytometric analysis of permeabilized HeLa cells using 1/10 ab109435 (red) or a rabbit IgG (negative) (green).
  • OI-RD Scanning - Anti-USP22 antibody [EPR4352(2)] (ab109435)
    OI-RD Scanning - Anti-USP22 antibody [EPR4352(2)] (ab109435)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-USP22 antibody [EPR4352(2)] (ab109435)
    Anti-USP22 antibody [EPR4352(2)] (ab109435)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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