Anti-BRCA1 (phospho S1423) antibody (ab90528)
Key features and details
- Rabbit polyclonal to BRCA1 (phospho S1423)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRCA1 (phospho S1423) antibody
See all BRCA1 primary antibodies -
Description
Rabbit polyclonal to BRCA1 (phospho S1423) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human BRCA1 aa 1400-1500 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104954) -
Positive control
- WB: Irradiated HeLa Whole Cell Lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Images
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Western blot - Anti-BRCA1 (phospho S1423) antibody (ab90528)All lanes : Anti-BRCA1 (phospho S1423) antibody (ab90528) at 1 µg/ml
Lane 1 : Irradiated HeLa Whole Cell Lysate
Lane 2 : Irradiated HeLa Whole Cell Lysate with Immunising Peptide at 1 µg/ml
Lane 3 : Irradiated HeLa Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 208 kDa
Observed band size: 208 kDa
Additional bands at: 110 kDa, 140 kDa, 160 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 (phospho S1423) antibody (ab90528)ICC/IF image of ab90528 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90528, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-BRCA1 (phospho S1423) antibody (ab90528) Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab90528 (1/200) staining BRCA1 (phospho S1423) in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
