Anti-APAF1 antibody [2H9A1] (ab233786)
![Anti-APAF1 antibody [2H9A1] (ab233786)](https://www.abcam.com/ps/products/233/ab233786/Images/ab233786-307380-anti-apaf1-antibody-2h9a1-western-blot.jpg "Anti-APAF1 antibody [2H9A1] (ab233786)")
Key features and details
- Mouse monoclonal [2H9A1] to APAF1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-APAF1 antibody [2H9A1]
See all APAF1 primary antibodies -
Description
Mouse monoclonal [2H9A1] to APAF1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human APAF1 aa 1100 to the C-terminus. (Expressed in E.coli).
Database link: O14727 -
Positive control
- WB: Recombinant human APAF1 (AA: 1138-1237) protein and APAF1 (AA: 1138-1237)-hIgGFc transfected HEK-293 whole cell lysate. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2H9A1 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-APAF1 antibody [2H9A1] (ab233786)Anti-APAF1 antibody [2H9A1] (ab233786) at 1/500 dilution + Recombinant human APAF1 (AA: 1138-1237) protein
Developed using the ECL technique.Expected MW is 37 kDa.
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![Western blot - Anti-APAF1 antibody [2H9A1] (ab233786)](https://www.abcam.com/ps/products/233/ab233786/Images/ab233786-307383-anti-apaf1-antibody-2h9a1-western-blot.jpg)
Western blot - Anti-APAF1 antibody [2H9A1] (ab233786)All lanes : Anti-APAF1 antibody [2H9A1] (ab233786) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : APAF1 (AA: 1138-1237)-hIgGFc transfected HEK-293 whole cell lysate
Developed using the ECL technique.
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![Immunocytochemistry/ Immunofluorescence - Anti-APAF1 antibody [2H9A1] (ab233786)](https://www.abcam.com/ps/products/233/ab233786/Images/ab233786-307384-anti-apaf1-antibody-2h9a1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-APAF1 antibody [2H9A1] (ab233786)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for APAF1 (green) using ab233786 at 1/100 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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![Flow Cytometry - Anti-APAF1 antibody [2H9A1] (ab233786)](https://www.abcam.com/ps/products/233/ab233786/Images/ab233786-307385-anti-apaf1-antibody-2h9a1-flow-cytometry.jpg)
Flow Cytometry - Anti-APAF1 antibody [2H9A1] (ab233786)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling APAF1 with ab233786 at 1/200 dilution (green) compared with a negative control (red).
