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Anti-ERCC1 antibody [EPR7062] (ab129093)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-ERCC1 antibody [EPR7062] (ab129093)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7062] to ERCC1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-ERCC1 antibody [EPR7062]
    See all ERCC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7062] to ERCC1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human ERCC1 aa 150-300. The exact sequence is proprietary.
    Database link: P07992

  • Positive control

    • HeLa, SKBR-3, and 293T cell lysates, Human lung carcinoma tissue, HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7062
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-ERCC1 antibody [EPR7062] (ab129093)
    Western blot - Anti-ERCC1 antibody [EPR7062] (ab129093)
    All lanes : Anti-ERCC1 antibody [EPR7062] (ab129093) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : SKBR-3 cell lysates
    Lane 3 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7062] (ab129093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7062] (ab129093)

    Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 with ab129093 (1/25 dilution) in immunohistochemical analysis.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7062] (ab129093)
    Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7062] (ab129093)
    ab129093, at a 1/100 dilution, staining ERCC1 in HeLa cells by Immunofluorescence.
  • Anti-ERCC1 antibody [EPR7062] (ab129093)
    Anti-ERCC1 antibody [EPR7062] (ab129093)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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