Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)
![Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)](https://www.abcam.com/ps/products/256/ab256417/Images/ab256417-2-AntiCD16CD32antibody93A488MouseC57BL6splenocytesflowcytometry.jpg "Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)")
Key features and details
- Alexa Fluor® 488 Rat monoclonal [93] to CD16+CD32
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Overview
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Product name
Alexa Fluor® 488 Anti-CD16+CD32 antibody [93]
See all CD16+CD32 primary antibodies -
Description
Alexa Fluor® 488 Rat monoclonal [93] to CD16+CD32 -
Host species
Rat -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC/IF Mouse
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Immunogen
Tissue, cells or virus corresponding to CD16+CD32. Mouse B cells
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Epitope
Reacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors. -
Positive control
- ICC/IF: RAW 264.7 cells. Flow Cytometry: C57 BL/6 mouse splenocytes.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
93 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)
Flow cytometry staining of C57 BL/6 mouse splenocytes stained with ab256417 (right) or Rat IgG2aκ Alexa Fluor® 488 isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256417) or Rat IgG2aκ Alexa Fluor® 488 isotype (1x 106 in 100 µl at 0.2 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD19.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live single cells.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)](https://www.abcam.com/ps/products/256/ab256417/Images/ab256417-1-anti-cd16-cd32-antibody-epr-immunocytochemistry-raw-264-7-mouse-a488.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)ab256417 staining CD16+CD32 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab256417 at 1/500 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Raw264.7 cells fixed with 4% formaldehyde (10 min).
