Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7277] to ERCC1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ERCC1 antibody [EPR7277] - BSA and Azide free
    See all ERCC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7277] to ERCC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human ERCC1 aa 1-200. The exact sequence is proprietary.
    Database link: P07992

  • Positive control

    • IHC-P: Human lung carcinoma tissue.

  • General notes

    ab232422 is the carrier-free version of ab129267 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232422 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)

    Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 with ab129267 (1/100 dilution) in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129267).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)
    Flow Cytometry - Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)

    Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling ERCC1 with purified ab129267 at 1/80 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129267).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)
    Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)
    ab129267, at 1/100 dilution, staining ERCC1 in HepG2 cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129267).

  • Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)
    Anti-ERCC1 antibody [EPR7277] - BSA and Azide free (ab232422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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