Anti-ERCC1 antibody (ab69398)
Key features and details
- Rabbit polyclonal to ERCC1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERCC1 antibody
See all ERCC1 primary antibodies -
Description
Rabbit polyclonal to ERCC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human ERCC1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab93790) -
Positive control
- This antibody gave a positive signal mouse kidney tissue lysate and in the following whole cell lysates: HeLa; Jurkat.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications HamsterCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
Involvement in disease
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
Sequence similarities
Belongs to the ERCC1/RAD10/SWI10 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 615637 Cow
- Entrez Gene: 2067 Human
- Entrez Gene: 13870 Mouse
- Omim: 126380 Human
- SwissProt: Q1LZ75 Cow
- SwissProt: P07992 Human
- SwissProt: P07903 Mouse
- Unigene: 435981 Human
see all -
Alternative names
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
see all
Images
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Western blot - Anti-ERCC1 antibody (ab69398)All lanes : Anti-ERCC1 antibody (ab69398) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 36 kDa, 55 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes -
Western blot - Anti-ERCC1 antibody (ab69398)Anti-ERCC1 antibody (ab69398) at 1 µg/ml + Kidney (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab69398 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ERCC1 antibody (ab69398)All lanes : Anti-ERCC1 antibody (ab69398) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 36 kDa, 55 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
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Western blot - Anti-ERCC1 antibody (ab69398)Anti-ERCC1 antibody (ab69398) at 1 µg/ml + Kidney (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
