Antibodies, Proteins, Kits and Reagents for Life Science

Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (ab259431)

Key features and details

Mouse monoclonal [MSH6/3086] to MSH6 - BSA and Azide free
Suitable for: WB, IHC-P, ICC/IF, Protein Array
Reacts with: Human
Isotype: IgG2b

Product name

Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free
See all MSH6 primary antibodies
Description

Mouse monoclonal [MSH6/3086] to MSH6 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Protein Arraymore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment within Human MSH6 aa 374-540. The exact sequence is proprietary.
Database link: P52701
Positive control
- WB: HCT 116 cell lysate. IHC-P: Human colon carcinoma and small intestine tissue. ICC/IF: MCF7 cells.
General notes

ab259431 is a PBS only version of ab259428.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from bioreactor concentrate.
Clonality

Monoclonal
Clone number

MSH6/3086
Isotype

IgG2b
Light chain type

kappa
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (ab259431)

PFA-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling MSH6 using ab259428 at 2 μg/ml (green) in ICC/IF.

The nuclear counterstain is Reddot (red).

The secondary antibody is a Goat anti-mouse IgG CF488 conjugate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (ab259431)

Formalin-fixed, paraffin-embedded human small intestine tissue labeling MSH6 using ab259428 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).
Western blot - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (ab259431)

Anti-MSH6 antibody [MSH6/3086] (ab259428) at 2 µg/ml + HCT 116 (human colorectal carcinoma cell line) cell lysate

Predicted band size: 153 kDa

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).
Protein Array - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (ab259431)

Protein array containing > 19,000 full-length human proteins, probed using ab259428.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (ab259431)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling MSH6 using ab259428 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com