Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)
Key features and details
- Goat polyclonal to Monoacylglycerol Lipase/MGL
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Monoacylglycerol Lipase/MGL antibody
See all Monoacylglycerol Lipase/MGL primary antibodies -
Description
Goat polyclonal to Monoacylglycerol Lipase/MGL -
Host species
Goat -
Specificity
abb77398 is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1). Preliminary testing was unsuccessful on Rat.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Monoacylglycerol Lipase/MGL aa 17-29 (internal sequence). (NP_001003794.1)
Sequence:C-QDLPHLVNADGQY
Database link: NP_009214.1
(Peptide available asab173372) -
General notes
This product was previously labelled as Monoacylglycerol Lipase
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77398 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)
Immunocytochemistry/immunofluorescence analysis of paraformaldehyde fixed U2OS cells labelling Monoacylglycerol Lipase/MGL with ab77398. Cells were permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (2 µg/mL).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemical staining of Monoacylglycerol Lipase/MGL using ab77398 on Formaldehyde-fixed paraffin-embedded mouse adipose tissue sections (from around the kidney). Antigen retrieval step: heat mediated in citric acid HIER. Blocking step: 1% BSA for 10 mins @ rt°C. ab77398 incubated at 1/500 for 2 hours. Secondary Antibody: biotin labelled anti goat IgG used at 1/200. The submitted image shows white fat deposits around the kidney. Dilution factors vary between 1/300-500. There is a pale positivity in the renal tubular cytoplasm which appears to be specific (at lower dilution factor this is more obvious but, in this block the fat was too strongly immunostained). Section was endogenous biotin-blocked prior to incubation with primary Ab.
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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)Anti-Monoacylglycerol Lipase/MGL antibody (ab77398) at 0.5 µg/ml + Mouse Adipose lysate (35 µg protein in RIPA buffer)
Predicted band size: 33 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Flow Cytometry - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398) -
Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)Immunocytochemistry/immunofluorescence analysis of paraformaldehyde fixed A431 cells labelling Monoacylglycerol Lipase/MGL with ab77398. Cells were permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL), showing cytoplasmic and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (2 µg/mL).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling Monoacylglycerol Lipase/MGL with ab77398 at 5 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. Counterstained with hematoxylin.
Inset: Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
